Enhanced engraftment of genetically modified bone marrow stromal cells by Jennifer Chu

Cover of: Enhanced engraftment of genetically modified bone marrow stromal cells | Jennifer Chu

Published by National Library of Canada in Ottawa .

Written in English

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Edition Notes

Thesis (M.Sc.) -- University of Toronto, 2001.

Book details

SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
Pagination1 microfiche : negative. --
ID Numbers
Open LibraryOL19081393M
ISBN 100612588513

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Introduction. Chemokine receptor CXCR4 and one of its two ligands, CXCL12 (also known as stromal cell derived factor 1 [SDF-1]), are involved in the migration, homing, mobilization, and survival of hematopoietic stem cells (HSCs) and hematopoietic progenitor cells Author: Hal E.

Broxmeyer. Cell Stem Cell Article Long-Term Engraftment of Primary Bone Marrow Stromal Cells Repairs Niche Damage and Improves Hematopoietic Stem Cell Transplantation Jean-Paul Abbuehl,1 Zuzana.

It is known that MSCs from bone marrow release growth factors such as epidermal growth factor, fibroblast growth factor, platelet-derived growth factor (PDGF), VEGF, and stromal cell-derived factor-1, all of which can direct and regulate endogenous fibroblasts and endothelial cells Cited by:   Bone marrow-derived human mesenchymal stem cells (hMSCs) and human embryonic stem cell-derived cells (hESdCs) were modified with angiogenic factor (VEGF) DNA by using poly(β Cited by: Chemokine receptor CXCR4 and one of its two ligands, CXCL12 (also known as stromal cell derived factor 1 [SDF-1]), are involved in the migration, homing, mobilization, and survival of hematopoietic stem cells (HSCs) and hematopoietic progenitor cells Author: Hal E.

Broxmeyer. The objective of this study was to determine the potential of human bone marrow derived mesenchymal stem cells (hBMSCs) as gene carriers for improving the outcome of human islet transplantation.

To obtain bone marrow stromal cells, we killed 4- to 6-week-old male mice and flushed bone marrow from the femur and tibia, and purified the marrow mononuclear cells by Ficoll gradient.

28 Bone. However, coinjections of bone marrow stromal cells seem to inhibit the proliferation of Kaposi's sarcoma cells. The effect of MSCs on tumor growth is therefore equivocal at this point, varying significantly by tumor cell.

Engraftment of the stem cells in the bone marrow can be seen as the end of a complex series of events in which circulating HSC are first recruited by the BM vasculature followed by their transendothelial migration into the extravascular hematopoietic cords of the marrow.

Bone marrow stromal cells (BMSCs) contains a subset of skeletal stem cells (SSCs) that are multipotent, capable of forming cartilage, bone, hematopoiesis-supportive stroma and marrow adipocytes.

SSCs have been identified as pericytes, cells on the abluminal side of marrow. A major impediment to the development of therapies with mesenchymal stem cells/multipotent stromal cells (MSC) is the poor survival and engraftment of MSCs at the site of.

@article{RoccoAnalysisOB, title={Analysis of Biodistribution and Engraftment into the Liver of Genetically Modified Mesenchymal Stromal Cells Derived from Adipose Tissue}, author={G.

Di Rocco and A. Gentile and A. Antonini and S. Truffa and G. Piaggio and M. Capogrossi and Gabriele Toietta}, journal={Cell. Human bone marrow stromal cells express a distinct set of biologically functional chemokine receptors.

Stem Cells. ; – Crossref Medline Google Scholar; 9 Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J. The in vitro migration capacity of human bone marrow mesenchymal stem cells.

Dazhi Yang, Shijin Sun, Zhengguo Wang, Peifang Zhu, Zailiang Yang, Bo Zhang, Stromal Cell-Derived Factor-1 Receptor CXCR4-Overexpressing Bone Marrow Mesenchymal Stem Cells Accelerate Wound Healing by Migrating into Skin Injury Areas, Cellular Reprogramming, /cell.

Analysis of Biodistribution and Engraftment Into the Liver of Genetically Modified Mesenchymal Stromal Cells Derived From Adipose Tissue a hepatic phenotype in of human bone marrow mesen-chymal cells (20). A method to induce hepatic differ- kinase promoter-enhanced.

Long-term engraftment of primary bone marrow stromal cells repairs niche damage and improves hematopoietic stem cell transplantation. Cell Stem Cell – Crossref, Medline, Google Scholar; 3. Yu SP, Wei Z and Wei L. Preconditioning strategy in stem cell. The use of stem cells for tissue regeneration and repair is advancing both at the bench and bedside.

Stem cells isolated from bone marrow are currently being tested for their therapeutic potential in a variety of clinical conditions including cardiovascular injury, kidney failure, cancer, and neurological and bone.

Bone marrow‐derived mesenchymal stromal cells (BM‐MSCs), as important stromal components of bone marrow, can support hematopoiesis and are multipotent stem cells with the capacity to differentiate into osteoblasts, chondrocytes, adipocytes, and neural cells.

Bone marrow stromal cells are progenitors of skeletal tissue components such as bone, cartilage, the hematopoiesis‐supporting stroma, and adipocytes. In addition, they may be experimentally induced to undergo unorthodox differentiation, possibly forming neural and myogenic cells.

Mesenchymal stem cells (MSCs) are currently being widely investigated both in the lab and in clinical trials for multiple disease states. The differentiation, trophic, and immunomodulatory characteristics of MSCs contribute to their therapeutic effects.

Another often overlooked factor related to efficacy is the degree of engraftment. When reported, engraftment. In this study, we investigated the feasibility of a plasmid-based strategy for in vitro genetic modification of human bone marrow-derived stromal cells (hMSC) with enhanced green fluorescent protein (EGFP) and NT3.

Abbuehl and colleagues show that irradiation required for hematopoietic stem cell (HSC) transplantation permanently damages the bone marrow (BM) stroma, which limits hematopoietic function. They develop protocols for intra-bone transplantation of multipotent BM stroma cells.

Circulating mesenchymal stem cells (MSCs) is a new cell source for tissue regeneration and tissue engineering. The characteristics of circulating MSCs are similar to those of bone marrow. Introduction. I n the field of cell therapy for regenerative medicine, progenitor/stem cells and informative and biopermissive scaffolds in which cells may grow are the two key players to repair the target tissue.

In this scenario, particularly in the orthopedic field, much is known on the long-term engraftment and regenerative potential of transplanted precursor cells. Next-Generation Stem Cell Therapy: Genetically Modified Mesenchymal Stem Cells for Cardiac Repair cardiac and bone marrow-derived mononuclear cells have been identified and evaluated for their regenerative potential for the treatment of heart disease.

PIM-1 kinase or stromal-derived factor-1 showed enhanced engraftment. Bone marrow cells (BMCs) could correct some pathologic conditions of the central nervous system (CNS) if these cells would effectively repopulate the brain.

One such condition is G. The effects of bone marrow stromal cells (BMSCs) on the repair of injured spinal cord and on the behavioral improvement were studied in the rat.

The spinal cord was injured by contusion using a weight-drop at the level of T, and the BMSCs from the bone marrow. Cell transplantation as a treatment for spinal cord injury is a promising therapeutic strategy whose effective clinical application would be facilitated by non-invasive delivery protocols.

Cells derived from the bone marrow. Vianello F, Villanova F, Tisato V, Lymperi S, Ho KK, Gomes AR, et al. Bone marrow mesenchymal stromal cells non-selectively protect chronic myeloid leukemia cells from imatinib. Hurwitz D.R., Kirchgesser M., Merrill W., et ic delivery of human growth hormone or human factor IX in dogs by reintroduced genetically modified autologous bone marrow stromal cells.

The potential of using bone marrow (BM)-derived human stromal cells for ex vivo gene therapy of hemophilia A was evaluated. BM stromal cells were transduced with an intron-based. Bone marrow cells can be enriched in MSCs by selecting for plastic-adherent cells.

These stromal cells will grow to confluency in appropriate culture conditions as flattened fibroblast-like cells (Friedenstein,   Mammalian bone marrow architecture involves haematopoietic stem cells (HSCs) in close proximity to the endosteal surfaces 5,6, with more differentiated cells arranged in a loosely.

Placenta-derived mesenchymal stem/stromal cells (PMSC) present several aspects that make them more attractive as cellular therapy than their counterparts from other tissues, such as MSC from bone marrow or adipose tissue in regenerative medicine.

Placenta-derived MSC have been used to treat a variety of disorders, such as, cancer, liver and cardiac diseases, ulcers, bone.

Cell culture. Rat bone marrow mesenchymal stem cells (rBMC) were harvested from 8 week young adult male Wistar rats.

Rats were sacrificed by cervical dislocation and the BM cells were harvested by flushing the femora with Dulbecco's modified. In a rat defect bone model, bone marrow mesenchymal stem cells over-expressing SDF-1 showed significantly (p=) more new bone formation within the gap and less bone mineral loss at the area.

Thus far, new strategy, such as genetic modification of MSCs, has been developed and has shown promising outcomes of various disorders. This study was conducted using integrin-linked kinase (ILK) gene-modified bone marrow-derived stem cells.

L'auteur conserve la propriété du droit d'auteur qui protège cette thèse. Ni la thèse ni des extraits substantiels de celle-ci ne doivent être imprimés ou autrement reproduits sans son autorisation. Enhanced engraftment of genetically modified bone marrow stromal cells.

In particular, adult stem cell-based therapies have been studied for several decades. Mesenchymal stem cells/marrow stromal cells (MSCs) have shown safety and therapeutic efficacy in preclinical models of various diseases such as cardiovascular disease, cancer, bone.

14 Eliopoulos N, Gagnon RF, Francois M, Galipeau J. Erythropoietin delivery by genetically engineered bone marrow stromal cells for correction of anemia in mice with chronic renal failure. J Am Soc.

Bone marrow stromal cells (BMSCs) are obtained by a simple bone marrow aspiration and can be expanded in vitro. They are not stem cells, but have many features of stem cells and can differentiate into several mature cell types, including cardiac muscles [ 8 ], pneumocysts [ 9 ], hepatocytes [ 10 ], neural cells.

Bone marrow is the soft, flexible connective tissue within bone cavities. A component of the lymphatic system, bone marrow functions primarily to produce blood cells and to store marrow is highly vascular, meaning that it is richly supplied with a large number of blood are two categories of bone marrow tissue: red marrow and yellow marrow.In this study, we performed cell implantation experiments in the CNS of immunocompetent mice using autologous (syngeneic) luciferase-expressing bone marrow-derived stromal cells (BMSC-Luc) cultured from ROSAL-S-L-Luciferase transgenic mice, and BMSC-Luc genetically modified using a lentivirus encoding the enhanced .

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